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Product Manual ViroReal® Kit Deformed Wing Virus |
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Material Safety Data Sheet ViroReal® Kit Deformed Wing Virus |
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Background: Deformed wing virus is a single-stranded RNA virus of the family Iflaviridae that causes deformed wings and abdomens of adult bees and shorten their lifespan dramatically. It is found worldwide and became one of the most important threat of honeybees and other pollinators. It has been often found as a coinfection with Varroa mites. The correlation of mite burden, virus load and severity of disease suggests that Varroa acts not only as a vector but also as a viral replicator. Other ways of transmission happen via feeding, and vertically through queen’s ovaria and drone sperm.
Description: ViroReal® Kit Deformed Wing Virus is a real-time PCR for the qualitative detection of the RNA of the polyprotein gene of deformed wing virus. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).
Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
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DVEV03813 | 100 | FAM | Cy5 | polyprotein gene |
DVEV03853 | 50 | FAM | Cy5 | polyprotein gene |
Shipping Temperature: -20 °C dry ice
PCR platforms: ViroReal® Deformed Wing Virus was validated with the QuantStudio™ 5 Real-Time PCR System (Thermo Fisher Scientific) and the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in FAM and Cy5 channel (eg. QuantStudio™ 5/6/7 Real-Time PCR System (Thermo Fisher Scientific), qTOWER3G (Analytik Jena), Mic instrument (bio moleclar systems), cobas z 480 Analyzer (Roche)).