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Product Manual ViroReal® Kit Deformed Wing Virus Download PDF
Material Safety Data Sheet ViroReal® Kit Deformed Wing Virus Download PDF
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Background:  Deformed wing virus is a single-stranded RNA virus of the family Iflaviridae that causes deformed wings and abdomens of adult bees and shorten their lifespan dramatically. It is found worldwide and became one of the most important threats to honeybees and other pollinators. It has often been found as a coinfection with Varroa mites. The correlation of mite burden, virus load and severity of disease suggests that Varroa acts not only as a vector but also as a viral replicator. Other ways of transmission happen via feeding, and vertically through queen’s ovaria and drone sperm.

Description:  ViroReal® Kit Deformed Wing Virus is a real-time PCR for the qualitative detection of the RNA of the polyprotein gene of deformed wing virus. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

ViroReal® Kit Deformed Wing Virus
Order Number Reactions Channel Pathogen Channel IPC Target
DVEV03813 100 FAM Cy5 polyprotein gene
DVEV03853 50 FAM Cy5 polyprotein gene
For veterinary use only
Shipping Temperature: -20 °C dry ice
Product Features:
  • Amplification and detection: RNA of the polyprotein of Deformed Wing Virus
  • Transcription with thermostable MMLV Reverse Transcriptase
  • Contains RNase Inhibitor to block RNA degradation
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix)

PCR platforms: ViroReal® Deformed Wing Virus was validated with the QuantStudio™ 5 Real-Time PCR System (Thermo Fisher Scientific), and was also tested with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in FAM and Cy5 channel (eg. QuantStudio™ 6 / 7 Real-Time PCR System (Thermo Fisher Scientific), qTOWER3G (Analytik Jena), Mic instrument (bio molecular systems), cobas z 480 Analyzer (Roche)).

Frequently Asked Questions:

The kit amplifies the polyprotein gene of DWV, and detection is performed in the FAM fluorescence channel.
Varroa mites are considered both vectors and viral amplifiers of DWV, significantly increasing virus load and disease severity in infected bee colonies.
Yes, the IPC can be added to the master mix at a 1:100 dilution to control for RT-PCR inhibition.
Validated instruments include QuantStudio 5/6/7, ABI 7500, qTOWER3G, MIC, and cobas z 480, supporting FAM and Cy5 detection.
Use separate workspaces for extraction and amplification, sterile RNase-free materials, and pipette the positive control last.
BactoReal®, MycoReal®, ParoReal® and ViroReal® Kits run with the same thermal cycling conditions.