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Product Manual BactoReal® Kit Corynebacterium pseudotuberculosis Download PDF
Material Safety Data Sheet BactoReal® Kit Corynebacterium pseudotuberculosis Download PDF
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Background:  Corynebacterium pseudotuberculosis is a Gram-positive bacterium and the aetiological agent of external or cutaneous abscessation, lymphangitis, and internal abscessation affecting sheep, goats, horses, cattle and rarely humans as well. Corynebacterium pseudotuberculosis produces an exotoxin called phospholipase D that enhances dissemination of the bacteria by damaging endothelial cells and increasing vascular permeability.

Description:  BactoReal® Kit Corynebacterium pseudotuberculosis is a real-time PCR for the qualitative detection of the phosholipase D (pld) gene of Corynebacterium pseudotuberculosis. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Corynebacterium pseudotuberculosis
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB05213 100 FAM Cy5 pld
DVEB05253 50 FAM Cy5 pld
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: pld gene of Corynebacterium pseudotuberculosis
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Corynebacterium pseudotuberculosis was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Corynebacterium pseudotuberculosis
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV919 100 FAM Cy5 pld
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The kit amplifies the phospholipase D (pld) gene, with detection in the FAM channel.
Infected animals may show external or internal abscessation, lymphangitis, or caseous lymphadenitis, reducing productivity and causing economic losses.
C. pseudotuberculosis DNA is detected in the FAM channel, while the IPC is detected in the Cy5 channel.
The kit was validated against multiple strains and is highly specific for C. pseudotuberculosis, with no relevant cross-reactivity observed.
Validated instruments include ABI 7500, LightCycler® 480 II, and Mx3005P®, as well as other systems detecting FAM and Cy5.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.