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Product Manual BactoReal® Kit Clostridium perfringens Download PDF
Material Safety Data Sheet BactoReal® Kit Clostridium perfringens Download PDF
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Background:  Clostridium perfringens is a spore-forming Gram-positive bacterium present in many environmental sources as well as in the intestines of humans and animals. Under certain predisposing conditions, Clostridium perfringens proliferates rapidly, produces toxins and causes disease such as gas gangrene, necrotic enteritis and enterotoxemia. This bacterium is an important cause of food poisoning. Stress and the feed composition are believed to be of major importance for the development of disease. Clostridium perfringens strains are classified into five types (A–E) based on their ability to produce the major lethal toxins alpha (cpa), beta (cpb), epsilon (etx), and iota (iap) which are responsible for the cytotoxic and necrotic activity associated with infection. In addition, strains of C. perfringens may also produce a number of other toxins, including enterotoxin (cpe), beta-2 toxin (cpb2) or theta toxin.

Description:  BactoReal® Kit Clostridium perfringens is a real-time PCR for the qualitative detection of the 16S rRNA gene of all five serotypes of Clostridium perfringens. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Clostridium perfringens
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB06513 100 FAM Cy5 16S
DVEB06553 50 FAM Cy5 16S
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: 16S rRNA gene of Clostridium perfringens
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Clostridium perfringens was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Clostridium perfringens
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV928 100 FAM Cy5 16S
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The kit specifically detects DNA of Clostridium perfringens by amplifying a region of the 16S rRNA gene.
C. perfringens can cause necrotic enteritis, gas gangrene, enterotoxemia, and foodborne illness in animals.
The kit includes an artificial DNA IPC to verify DNA extraction quality and identify possible PCR inhibition.
The kit is suitable for DNA extracted from stool or tissue samples.
Validation confirmed a sensitivity of about 5 DNA copies per reaction and high specificity through carefully selected primers and probes.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.