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Product Manual ViroReal® Kit Chronic Bee Paralysis Virus Download PDF
Material Safety Data Sheet ViroReal® Kit Chronic Bee Paralysis Virus Download PDF
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Background: 

Chronic bee paralysis virus (CBPV) is an enveloped virus with a genome consisting of three single plus-stranded RNA molecules. It commonly affects the honeybee Apis mellifera. The unclassified virus is phylogenetically placed between the Nodaviridae (insect and fish viruses) and Tombusviridae (plant viruses) family. It is suggested that CBPV is the prototype of a new family of positive single-stranded RNA viruses. Infections were rather unusual but a continuous increase of incidents worldwide was reported recently. The very characteristic symptoms like inability to fly, trembling, ataxia, and shiny hairless bodies could even lead to collapses of the bee colonies. Infected symptomatic bees, mostly adults, die within a few days.

Description: 

ViroReal® Kit Chronic Bee Paralysis Virus is a real-time PCR for the qualitative detection of the RNA of the RdRp gene of chronic bee paralysis virus. Our carefully designed primers and probes ensure the highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

ViroReal® Kit Chronic Bee Paralysis Virus
Order Number Reactions Channel Pathogen Channel IPC Target
DVEV04213 100 FAM Cy5 RdRp gene
DVEV04253 50 FAM Cy5 RdRp gene
For veterinary use only
Shipping Temperature: -20 °C dry ice
Product Features:
  • Amplification and detection: RdRp gene of Chronic Bee Paralyis Virus
  • Transcription with thermostable MMLV Reverse Transcriptase
  • Contains RNase Inhibitor to block RNA degradation
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix)

PCR platforms:

ViroReal® Chronic Bee Paralysis Virus was validated with the QuantStudio™ 5 Real-Time PCR System (Thermo Fisher Scientific), and was also tested with ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 6 / 7 (Thermo Fisher Scientific), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), and cobas z 480 Analyzer (Roche)).

Frequently Asked Questions:

The kit is designed for qualitative detection of RNA from Chronic Bee Paralysis Virus (CBPV) by targeting the RdRp gene using real-time RT-PCR.
CBPV causes trembling, inability to fly, ataxia, and hairless shiny abdomens. Infected adult bees often die within a few days, and outbreaks may lead to colony collapse.
The kit includes an artificial RNA internal positive control (IPC) added during extraction to monitor RNA integrity and PCR inhibition, detected in the Cy5 channel.
The limit of detection (LoD95%) is approximately 2.5 RNA copies per reaction.
The kit is validated for RNA extracted from honeybee colony material, including homogenized bee samples.
BactoReal®, MycoReal®, ParoReal® and ViroReal® Kits run with the same thermal cycling conditions.