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Product Manual ViroReal® Kit Chronic Bee Paralysis Virus |
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Material Safety Data Sheet ViroReal® Kit Chronic Bee Paralysis Virus |
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Background:
Chronic bee paralysis virus (CBPV) is an enveloped virus with a genome consisting of three single plus-stranded RNA
molecules. It commonly affects the honeybee Apis mellifera. The unclassified virus is phylogenetically placed between
the Nodaviridae (insect and fish viruses) and Tombusviridae (plant viruses) family. It is suggested that CBPV is the
prototype of a new family of positive single-stranded RNA viruses. Infections were rather unusual but a continuous
increase of incidents worldwide was reported recently. The very characteristic symptoms like inability to fly, trembling,
ataxia, and shiny hairless bodies could even lead to collapses of the bee colonies. Infected symptomatic bees, mostly
adults, die within a few days.
Description:
ViroReal® Kit Chronic Bee Paralysis Virus is a real-time PCR for the qualitative detection of the RdRp gene of chronic bee paralysis virus. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).
ViroReal® Kit Chronic Bee Paralysis Virus
Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
---|---|---|---|---|
DVEV04213 | 100 | FAM | Cy5 | RdRp gene |
DVEV04253 | 50 | FAM | Cy5 | RdRp gene |
Shipping Temperature: -20 °C dry ice
Product Features:
Amplification and detection: RdRp gene of Chronic Bee Paralyis Virus Real-time PCR with rapid hot-start Taq DNA polymerase ROX™ dye as passive reference Internal Positive Control System to exclude false-negative results Optimized to handle PCR inhibitors PCR- platforms: runs on all established standard real-time PCR- platforms Harmonized thermal profiles to run RNA and DNA samples simultaneously
PCR platforms:
ViroReal® Chronic Bee Paralysis Virus was validated with the QuantStudio™ 5 Real-Time PCR System (Thermo Fisher Scientific) and the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in FAM and Cy5 channel (eg. QuantStudio™ 5/6/7 Real-Time PCR System (Thermo Fisher Scientific), qTOWER3G (Analytik Jena), Mic instrument (bio moleclar systems), cobas z 480 Analyzer (Roche)).
BactoReal®, MycoReal®, ParoReal® and ViroReal® Kits run with the same thermal cycling conditions.