Back
Inquiry

Download

Product Manual BactoReal® Kit Chlamydiaceae Download PDF
Material Safety Data Sheet BactoReal® Kit Chlamydiaceae Download PDF
Download All

Background:  The Chlamydiaceae family currently includes two genera and one candidate genus: genus Chlamydia (including the species Chlamydia muridarum, Chlamydia suis, Chlamydia trachomatis), genus Chlamydophila (including the species Chlamydophila abortus, Chlamydophila caviae Chlamydophila felis, Chlamydia pecorum, Chlamydophila pneumoniae, Chlamydophila psittaci), and candidatus Clavochlamydia. All Chlamydiaceae are obligate intracellular Gram-negative bacteria that cause diseases in humans and animals worldwide.

Description:  BactoReal® Kit Chlamydiaceae is a real-time PCR for the qualitative detection of the 23S rRNA gene of C. muridarum, C. suis, C. trachomatis, C. abortus, C. caviae, C. felis, C. pecorum, C. pneumoniae and C. psittaci. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Chlamydiaceae
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB03113 100 FAM Cy5 23S rRNA
DVEB03153 50 FAM Cy5 23S rRNA
For research use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: 23S rRNA gene of Chlamydiaceae
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Chlamydiaceae was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Chlamydiaceae
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV1400 100 FAM Cy5 23S rRNA
For research use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The kit detects members of the Chlamydiaceae family, including Chlamydia trachomatis, Chlamydophila abortus, C. psittaci and related strains.
The kit amplifies the 23S rRNA gene, which is highly conserved among Chlamydiaceae species.
The IPC monitors DNA extraction efficiency and identifies potential PCR inhibition to ensure reliable test results.
Validated samples include swabs and tissue material from suspected infections.
The kit detects as few as 10 target DNA copies per reaction, with a 95% detection limit of 73 copies per reaction.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.