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Product Manual ViroReal® Kit CSF Virus Download PDF
Material Safety Data Sheet ViroReal® Kit CSF Virus Download PDF
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Background:  The CSF (classical swine fever virus) virus is a single strain, positive-sense ((+)sRNA) RNA virus belonging to the genus Pestivirus in the family Flaviviridae. This virus is closely related to ruminant pestiviruses that cause bovine viral diarrhoea (BVDV) and border disease (BDV) of sheep, both of which can also infect pigs naturally causing reproductive problems. The CSF virus is the infectious agent of classical swine fever or hog cholera (also sometimes called pig plague based on the German word “Schweinepest”), which is a highly contagious disease of swine. Swine fever virus causes fever, skin lesions, convulsions, and usually death. The effect of different CSF virus strains varies widely, leading to a wide range of clinical signs. There is only one serotype of the virus and attenuated vaccines are available.

Description:  ViroReal® Kit CSF Virus uses one-step reverse transcription RT real-time PCR for the detection of the 5´UTR of the CSF virus genome. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.

ViroReal® Kit CSF Virus
Order Number Reactions Channel Pathogen Channel IPC Target
DVEV02313 100 FAM Cy5 5´UTR
DVEV02353 50 FAM Cy5 5´UTR
For veterinary use only
Shipping Temperature: -20 °C dry ice
Product Features:
  • Amplification and detection: 5´UTR of CSF Virus
  • Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
  • contains RNase Inhibitor to block RNA degradation
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle RT-PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ViroReal® Kit CSF Virus is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® CSF Virus
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV26 100 FAM Cy5 5´UTR
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The kit amplifies the 5′UTR of CSFV RNA and detects fluorescence in the FAM channel.
The IPC helps confirm successful RNA extraction and checks for reverse transcription or PCR inhibition, reducing false-negative results.
Validated instruments include ABI 7500, LightCycler 480 II, Mx3005P, QuantStudio 5/6/7, MIC, qTOWER3G, and cobas z 480.
Yes, the IPC RNA can be added during extraction or directly into the master mix to control RT-PCR only.
The kit cross-reacts with BVDV and BDV, both of which can naturally infect pigs and cause reproductive disorders.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.