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Product Manual BactoReal® Kit Brachyspira hyodysenteriae Download PDF
Material Safety Data Sheet BactoReal® Kit Brachyspira hyodysenteriae Download PDF
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Background:  Brachyspira hyodysenteriae (previously called Serpulina hyodysenteriae or Treponema hyodysenteriae) is an intestinal spirochaete and the causative agent of swine dysentery. Swine dysentery is a disease characterized by mucohaemorrhagic diarrhoea with lesions confined to the large intestine of pigs. Its incubation time is 10-14 days. The faecal excretion of B. hyodysenteriae starts 2 days after infection. Brachyspira hyodysenteriae is an anaerobic bacterium but is aerotolerant due, at least in part, to high NADH oxidase activity.

Description:  BactoReal® Kit Brachyspira hyodysenteriae is a real-time PCR for the qualitative detection of the nox gene (NADH oxidase) of B. hyodysenteriae. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Brachyspira hyodysenteriae
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB01113 100 FAM Cy5 nox
DVEB01153 50 FAM Cy5 nox
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: nox gene (NADH oxidase) of Brachyspira hyodysenteriae
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Brachyspira hyodysenteriae was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Brachyspira hyodysenteriae
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV610 100 FAM Cy5 nox
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The kit targets the nox gene of B. hyodysenteriae, enabling specific detection of this swine pathogen by real-time PCR.
B. hyodysenteriae is the primary cause of swine dysentery, a severe intestinal infection characterized by mucohaemorrhagic diarrhoea and high economic impact.
Validated samples include DNA extracts from pig faeces and intestinal biopsies, the standard materials for diagnosing swine dysentery.
An internal DNA positive control (IPC), detected in the Cy5 channel, is added during DNA extraction to monitor both extraction efficiency and PCR inhibition.
The kit detects as few as 10 DNA copies per PCR reaction, with a limit of detection (LoD95%) of 18 copies per reaction and no cross-reactivity with non-target organisms.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.