Back
Inquiry

Download

Product Manual BactoReal® Kit Brachyspira hyo/pilo Download PDF
Material Safety Data Sheet BactoReal® Kit Brachyspira hyo/pilo Download PDF
Download All

Background:  Brachyspira are anaerobic intestinal spirochaetes. Brachyspira hyodysenteriae (previously called Serpulina hyodysenteriae or Treponema hyodysenteriae) is the causative agent of swine dysentery. Swine dysentery is a disease characterized by mucohaemorrhagic diarrhoea with lesions confined to the large intestine of pigs. Its incubation time is 10–14 days. The faecal excretion of B. hyodysenteriae starts 2 days after infection. Brachyspira pilosicoli (previously called Serpulina pilosicoli) colonizes the large intestine of various species, including humans. It is the etiologic agent of human and animal intestinal spirochetosis and rarely implicated as a cause of bacteremia. For example, it is the causative agent of porcine colonic spirochaetosis (PCS). Brachyspira are anaerobic bacteria but are aerotolerant due, at least in part, to high NADH oxidase activity.

Description:  BactoReal® Kit Brachyspira hyo/pilo is a real-time PCR for the qualitative detection of the NADH oxidase (nox) gene of B. hyodysenteriae and B. pilosicoli. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Brachyspira hyo/pilo
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB01813 100 FAM Cy5 nox
DVEB01853 50 FAM Cy5 nox
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: NADH nox gene of B. hyodysenteriae and B. pilosicoli
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Brachyspira hyo/pilo was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Brachyspira hyo/pilo
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV600 100 FAM Cy5 nox
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The kit detects DNA of Brachyspira hyodysenteriae, the causative agent of swine dysentery, and Brachyspira pilosicoli, responsible for porcine colonic spirochetosis and intestinal spirochetosis in various species.
The kit amplifies the nox gene of Brachyspira species, enabling species-specific detection via real-time PCR.
Validated specimens include DNA extracts from faeces and intestinal biopsies.
The internal DNA positive control (IPC), added during DNA extraction and detected in the Cy5 channel, verifies extraction efficiency and rules out PCR inhibition.
The analytical sensitivity is 10 copies per reaction, with a LoD95% of 18 copies for B. hyodysenteriae and 25 copies for B. pilosicoli. Validation confirmed 100% specificity without cross-reactions to other tested bacteria.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.