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Product Manual ViroReal® Kit Bovine Torovirus Download PDF
Material Safety Data Sheet ViroReal® Kit Bovine Torovirus Download PDF
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Background:  The species bovine torovirus (BToV) is a member of the family Tobaniviridae, subfamily Torovirinae. The genome is a single-stranded, positive-sense RNA molecule. The kidney-shaped BToV causes diarrhea in calves but respiratory infections have also been reported and it has a worldwide distribution. Bovine torovirus, formerly called breda virus (BRV), was originally isolated from diarrheic calves in Breda, Iowa, in 1979.

Description:  ViroReal® Kit Bovine Torovirus is a real-time PCR for the qualitative detection of the membrane protein (M) gene of the BToV. Our carefully designed primers and probes ensure the highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

ViroReal® Kit Bovine Torovirus
Order Number Reactions Channel Pathogen Channel IPC Target
DVEV01613 100 FAM Cy5 membrane protein M gene
DVEV01653 50 FAM Cy5 membrane protein M gene
For veterinary use only
Shipping Temperature: -20 °C dry ice
Product Features:
  • Amplification and detection: M gene of Bovine Torovirus
  • Transcription with thermostable MMLV Reverse Transcriptase
  • Contains RNase Inhibitor to block RNA degradation
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle RT-PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ViroReal® Kit Bovine Torovirus is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® Bovine Torovirus
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV90 100 FAM Cy5 membrane protein M gene
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The kit targets the membrane protein (M) gene of BToV RNA, which is detected using a FAM-labeled probe.
The IPC helps verify the success of RNA extraction and identifies RT-PCR inhibition, reducing the risk of false-negative results.
The kit is validated for ABI 7500, LightCycler 480 II, Mx3005P, QuantStudio 5/6/7, MIC, qTOWER3G, and cobas z 480.
Yes. The IPC RNA can be added during extraction for full-process monitoring or into the PCR master mix to monitor RT-PCR performance only.
Primers and probes were validated in silico and experimentally to detect all known BToV strains without cross-reactivity.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.