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| Product Manual ViroReal® Kit Bovine Parainfluenza Virus 3 |
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| Material Safety Data Sheet ViroReal® Kit Bovine Parainfluenza Virus 3 |
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Background: Bovine Parainfluenza Virus 3 (BPIV3) is an enveloped, single-stranded, negative-sense RNA ((-)ssRNA) virus belonging to the Paramyxoviridae family and is genetically and antigenically related to Human Parainfluenza Virus 3. Three genotypes, A (BPIV3a), B (BPIV3b), and C (BPIV3c), have been described, based on genetic and phylogenetic analysis. BPIV3 infections are found in a wide variety of mammals, including cattle, humans, sheep, goats, bison, guinea pigs, rhinoceros, moose, bighorn sheep, and camels. Cross-species infections have been reported. Usually, viral infection is asymptomatic or associated with only mild respiratory symptoms. In cattle, it is associated with the clinical picture known as shipping fever. It is an important virus of the bovine respiratory disease complex. When coinfection with other pneumonia-associated agents (e.g. respiratory syncytial virus, bacteria) is present, severe respiratory disease may develop.
Description: ViroReal® Kit Bovine Parainfluenza Virus 3 is a real-time PCR for the qualitative detection of the RNA of the phosphoprotein P gene of the so far known Bovine Parainfluenza Virus 3 (BPIV3) genotypes A, B and C. Our carefully designed primers and probes ensure the highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DVEV02813 | 100 | FAM | Cy5 | phosphoprotein P gene |
| DVEV02853 | 50 | FAM | Cy5 | phosphoprotein P gene |
Shipping Temperature: -20 °C dry ice
- Amplification and detection: RNA of phosphoprotein P of Bovine Parainfluenza Virus 3
- Transcription with thermostable MMLV Reverse Transcriptase
- Contains RNase Inhibitor to block RNA degradation
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference • Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR platforms: runs on all established standard real-time PCR platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Available formats: Kit (with reaction mix) or Assay-only
PCR platforms: ViroReal® Kit Bovine Parainfluenza Virus 3 was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent) and LightCycler® 480 II (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), cobas z 480 Analyzer (Roche), and QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).
Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| RTGV24 | 100 | FAM | Cy5 | phosphoprotein P gene |
Shipping Temperature: -20 °C to +4 °C