Back
Inquiry

Download

Product Manual ViroReal® Kit Bovine Norovirus Download PDF
Material Safety Data Sheet ViroReal® Kit Bovine Norovirus Download PDF
Download All

Background:  Noroviruses are non-enveloped, single-stranded, positive-sense RNA viruses ((+)ssRNA), belonging to the family Caliciviridae. Strains are classified in five genotypes (GI – GV), infecting different species, including human, bovine, swine, mice and oysters. Bovine noroviruses belong to GIII. Norovirus GIII includes two genotypes (GIII.1 and GIII.2), with genotype GIII.1 represented by jena virus and GIII.2 represented by newbury-2 virus. Infection is associated with gastrointestinal manifestations such as diarrhea, villus atrophy and crypt hyperplasia of the proximal small intestine in cattle, especially in young calves.

Description:  ViroReal® Kit Bovine Norovirus is a real-time PCR for the qualitative detection of the RNA of the ORF1 of all known Bovine Norovirus (BNoV) genotypes (GIII.1 and GIII.2). Our carefully designed primers and probes ensure the highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

ViroReal® Kit Bovine Norovirus
Order Number Reactions Channel Pathogen Channel IPC Target
DVEV02613 100 FAM Cy5 ORF 1
DVEV02653 50 FAM Cy5 ORF 1
For veterinary use only
Shipping Temperature: -20 °C dry ice
Product Features:
  • Amplification and detection: RNA of ORF1 of Bovine Norovirus
  • Transcription with thermostable MMLV Reverse Transcriptase
  • Contains RNase Inhibitor to block RNA degradation
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ViroReal® Kit Bovine Norovirus wurde für die Verwendung mit folgenden Geräten entwickelt: ABI® 7500 Fast Real-Time PCR System (fast cycle parameters werden nicht unterstützt, Thermo Fisher Scientific). Er eignet sich auch für andere real-time PCR-Geräte, die Fluoreszenz im FAM und Cy5 Kanal messen und differenzieren können (z.B. Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche), cobas z 480 Analyzer (Roche) und QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® Bovine Norovirus
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV21 100 FAM Cy5 ORF 1
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The kit targets the ORF1 region of BNoV RNA and detects amplification in the FAM channel.
The kit detects BNoV genotypes GIII.1 (Jena virus) and GIII.2 (Newbury-2 virus).
The RNA IPC confirms efficient RNA extraction and absence of RT-PCR inhibition, reducing false-negative results.
Validated instruments include ABI 7500, LightCycler 480 II, Mx3005P, QuantStudio 5/6/7, MIC, qTOWER3G, and cobas z 480.
Primers and probes were validated in silico and experimentally to detect all known BNoV strains without cross-reactivity.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.