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Product Manual BactoReal® Kit Borrelia burgdorferi sensu lato Download PDF
Material Safety Data Sheet BactoReal® Kit Borrelia burgdorferi sensu lato Download PDF
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Background:  Borrelia are Gram-negative bacteria belonging to the Spirochaetaceae. Borrelia burgdorferi sensu stricto, B. garinii, B. afzelii, B. valaisiana, B. lusitaniae and B. spielmanii belong to the B. burgdorferi sensu lato-complex and cause Lyme disease (Lyme borreliosis). These species are associated with different genospecies specific symptoms. They are transmissed by ticks, mainly by Ixodes ricinus. Lyme disease is a multisystemic illness, which can affect various organs, especially the nervous system, skin, heart and joints.

Description:  BactoReal® Kit Borrelia burgdorferi sensu lato is a real-time PCR for the qualitative detection of the ospA gene of Borrelia species belonging to the Borrelia burgdorferi sensu lato complex. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Borrelia burgdorferi sensu lato
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB05013 100 FAM Cy5 ospA gene
DVEB05053 50 FAM Cy5 ospA gene
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: ospA gene of Borrelia burgdorferi sensu lato
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Borrelia burgdorferi sensu lato was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Borrelia burgdorferi sensu lato (ospA)
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV1270 100 FAM Cy5 ospA gene
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The kit amplifies the ospA gene, detected in the FAM channel.
The IPC monitors DNA extraction efficiency and identifies possible PCR inhibition, ensuring accurate results.
No cross-reactions were observed with bacteria such as Bordetella, Chlamydophila, Haemophilus, Legionella, Listeria, Mycoplasma, Neisseria, Leptospira or Treponema.
It is the causative agent of Lyme disease, which can manifest with erythema migrans, arthritis, carditis, and neurological symptoms.
Validated instruments include ABI 7500, LightCycler® 480 II, and Mx3005P®, with compatibility for other systems detecting FAM and Cy5 fluorescence.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.