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Product Manual BactoReal® Kit Bordetella bronchiseptica Download PDF
Material Safety Data Sheet BactoReal® Kit Bordetella bronchiseptica Download PDF
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Background:  Bordetella bronchiseptica is a Gram-negative bacterium found as a normal inhabitant of the respiratory tract in a variety of animals such as dogs, pigs, some rodents, as well as humans. In some cases, it causes pneumonia, canine infectious tracheobronchitis (kennel cough), bronchopneumonia in cats, and atrophic rhinitis in pigs. It is widely distributed in the swine population. Here, some strains cause a mild and non-progressive rhinitis that heals spontaneously. However, if toxigenic pasteurella are present in the herd, then a combination of the two organisms can produce severe progressive rhinitis (PAR). Bordetella bronchiseptica can also be a secondary opportunist invader in pneumonia.

Description:  BactoReal® Kit Bordetella bronchiseptica is a real-time PCR for the qualitative detection of the bfrA gene of Bordetella bronchiseptica. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination, the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Bordetella bronchiseptica
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB02413 100 FAM Cy5 bfrA gene
DVEB02453 50 FAM Cy5 bfrA gene
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: bfrA gene of Bordetella bronchiseptica
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms:

BactoReal® Kit Bordetella bronchiseptica was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Bordetella bronchiseptica
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV1250 100 FAM Cy5 bfrA gene
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The kit amplifies the bfrA gene of Bordetella bronchiseptica, with detection in the FAM channel of real-time PCR instruments.
B. bronchiseptica can cause kennel cough in dogs, bronchopneumonia in cats, pneumonia in various species, and atrophic rhinitis in pigs. It may also act as a secondary invader in respiratory infections.
The kit is validated for DNA extracts from respiratory tract samples such as nasal swabs and lung tissue.
An internal DNA positive control (IPC) is added during DNA extraction and detected in the Cy5 channel to verify DNA extraction quality and detect PCR inhibition.
The kit has an analytical sensitivity of 1 DNA copy per PCR and was validated against multiple bacterial species without showing cross-reactivity.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.