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Product Manual ViroReal® Kit Black Queen Cell Virus Download PDF
Material Safety Data Sheet ViroReal® Kit Black Queen Cell Virus Download PDF
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Background: 

Black queen cell virus (BQCV) is a non-enveloped single-stranded RNA virus and belongs to the Dicistroviridae family and to the genus Triatovirus. The virus is the most common cause of death in queen´s larvae and pupae after sealing their cells, especially of A. mellifera, A. florea and A. dorsata. Viral infection turn the cell wall´s and brood´s color into brown and black. The virus is transmitted through feeding of the larvae, from hive to hive and via Nosema apis as a parasitic vector. Infection of queens, young workers or adult bees could happen but without visible symptoms.

Description: 

ViroReal® Kit Black Queen Cell Virus is a real-time PCR for the qualitative detection of the RNA of the the capsid protein gene of Black Queen Cell Virus (BQCV). Our carefully designed primers and probes ensure the highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

ViroReal® Kit Black Queen Cell Virus
Order Number Reactions Channel Pathogen Channel IPC Target
DVEV04413 100 FAM Cy5 capsid protein gene
DVEV04453 50 FAM Cy5 capsid protein gene
For veterinary use only
Shipping Temperature: -20 °C dry ice
Product Features:
  • Amplification and detection: RNA of capsid protein of Black Queen Cell Virus
  • Transcription with thermostable MMLV Reverse Transcriptase
  • Contains RNase Inhibitor to block RNA degradation
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix)

PCR platforms:

ViroReal® Black Queen Cell Virus was validated with the QuantStudio™ 5 Real-Time PCR System (Thermo Fisher Scientific), and was also tested with ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 6 / 7 (Thermo Fisher Scientific), Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), and cobas z 480 Analyzer (Roche)).

Frequently Asked Questions:

The kit targets the capsid protein gene of BQCV, and detection is carried out in the FAM fluorescence channel.
BQCV causes color changes in brood and cell walls (brown/black), especially affecting queen pupae. Adult bees may remain asymptomatic despite infection.
Yes, if not used during extraction, the IPC can be diluted 1:100 and added directly to the master mix before amplification.
The kit is compatible with QuantStudio 5/6/7, ABI 7500, qTOWER3G, MIC, cobas z 480, and others capable of detecting FAM and Cy5 fluorescence.
Use separate areas for RNA extraction and PCR setup, RNase-free materials, and always pipette the positive control last.
BactoReal®, MycoReal®, ParoReal® and ViroReal® Kits run with the same thermal cycling conditions.