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Product Manual ViroReal® Kit Black Queen Cell Virus |
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Material Safety Data Sheet ViroReal® Kit Black Queen Cell Virus |
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Background:
Black queen cell virus (BQCV) is a non-enveloped single-stranded RNA virus and belongs to the Dicistroviridae family and to the genus Triatovirus. The virus is the most common cause of death in queen´s larvae and pupae after sealing their cells, especially of A. mellifera, A. florea and A. dorsata. Viral infection turn the cell wall´s and brood´s color into brown and black. The virus is transmitted through feeding of the larvae, from hive to hive and via Nosema apis as a parasitic vector. Infection of queens, young workers or adult bees could happen but without visible symptoms.
Description:
ViroReal® Kit Black Queen Cell Virus is a real-time PCR for the qualitative detection of the RNA of the capsid protein gene of Black Queen Cell Virus. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).
ViroReal® Kit Black Queen Cell Virus
Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
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DVEV04413 | 100 | FAM | Cy5 | capsid protein gene |
DVEV04453 | 50 | FAM | Cy5 | capsid protein gene |
Shipping Temperature: -20 °C dry ice
Product Features:
Amplification and detection: RNA of capsid protein of Black Queen Cell Virus Real-time PCR with rapid hot-start Taq DNA polymerase ROX™ dye as passive reference Internal Positive Control System to exclude false-negative results Optimized to handle PCR inhibitors PCR- platforms: runs on all established standard real-time PCR- platforms Harmonized thermal profiles to run RNA and DNA samples simultaneously
PCR platforms:
ViroReal® Black Queen Cell Virus was validated with the QuantStudio™ 5 Real-Time PCR System (Thermo Fisher Scientific) and the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in FAM and Cy5 channel (eg. QuantStudio™ 5/6/7 Real-Time PCR System (Thermo Fisher Scientific), qTOWER3G (Analytik Jena), Mic instrument (bio moleclar systems), cobas z 480 Analyzer (Roche)).
BactoReal®, MycoReal®, ParoReal® and ViroReal® Kits run with the same thermal cycling conditions.