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Product Manual BactoReal® Kit Bartonella spp. Download PDF
Material Safety Data Sheet BactoReal® Kit Bartonella spp. Download PDF
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Background:  Bartonella species are Gram-negative bacteria. In addition to dogs and cats, numerous domestic and wild animals, including bovine, canine, and rodent species as well as humans can serve as a reservoir host for various Bartonella species. Cats may harbor B. henselae, B. clarridgeiae and rarely other Bartonella species. Dogs may carry Bartonella vinsonii subsp. berkhoffii, Bartonella henselae and others.

Description:  BactoReal® Kit Bartonella spp. is a real-time PCR for the qualitative detection of the gltA gene of Bartonella species. This kit is specific for B. clarridgeiae, B. elizabethae, B. grahamii, B. henselae, B. koehlerae, B. quintana, B. volans and B. washoensis. Bartonella rochalimae, B. vinsonii and B. bovis are detected with very low sensitivity. Bartonella bacilliformis is not detected. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Bartonella spp.
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB03913 100 FAM Cy5 gltA gene
DVEB03953 50 FAM Cy5 gltA gene
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: gltA gene of Bartonella spp.
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Bartonella spp. was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Bartonella spp.
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV1260 100 FAM Cy5 gltA gene
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The kit amplifies the gltA gene for reliable detection of Bartonella DNA.
An internal positive control (IPC) is included to monitor DNA extraction and identify PCR inhibition.
Cats, dogs, rodents, and various wild and domestic animals can serve as reservoirs; humans can also act as incidental hosts.
Bartonella DNA is detected in the FAM channel, and the IPC is detected in the Cy5 channel.
No, Bartonella bacilliformis is not detected by this kit.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.