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Product Manual ViroReal® Kit BVD Virus Download PDF
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Background:  The genus Pestivirus is composed of 4 important pathogens: bovine viral diarrhea virus 1 and 2 (BVDV-1 and BVDV-2), classical swine fever virus (CSFV), and border disease virus of sheep (BDV). Recently, a new putative pestivirus species called “HoBi-like,” “BVDV-3,” or “atypical pestivirus” was identified. The BVD virus is a single-strand, positive-sense RNA virus found throughout the world and causes bovine viral diarrhea (BVD). BVD is a disease of cattle, which causes various clinical symptoms, including diarrhea, mucosal disease, reproductive dysfunctions, and/or hemorrhagic syndrome. Prenatal infection with BVDV results in persistently infected cattle (BVD-PI). These animals carry the virus throughout their lifetime and secrete a large number of viral particles.

Description:  ViroReal® Kit BVD Virus is a real-time PCR for the qualitative detection of the RNA of the 5´UTR of BVDV-1, BVDV-2 and BVDV-3. Our carefully designed primers and probes ensure the highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

Upon request, ​ViroReal® Kit BVD can be expanded with additional primer pairs in order to detect also border disease virus (BDV) of sheep and CSFV-like (classical swine fever virus) pestivirus of ruminants:

Expansion primer for BDV - order no. ADD01

Expansion primer for CSFV - order no. ADD02

ViroReal® Kit BVD Virus
Order Number Reactions Channel Pathogen Channel IPC Target
DVEV00613 100 FAM Cy5 5´UTR
DVEV00653 50 FAM Cy5 5´UTR
For veterinary use only
Shipping Temperature: -20 °C dry ice
Product Features:
  • Amplification and detection: 5´UTR of BVD Virus
  • Transcription with thermostable MMLV Reverse Transcriptase
  • Contains RNase Inhibitor to block RNA degradation
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle RT-PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix)

PCR platforms: ViroReal® Kit BVD Virus was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent) and LightCycler® 480 II (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific), qTOWER3G (Analytik Jena), Mic instrument (bio molecular systems), cobas z 480 Analyzer (Roche)).

Frequently Asked Questions:

The kit targets the highly conserved 5′ untranslated region (5′UTR) of BVDV RNA, enabling broad strain detection.
BVDV causes various clinical syndromes in cattle including diarrhea, mucosal disease, reproductive disorders, and hemorrhagic syndromes.
The RNA IPC confirms both extraction efficiency and RT-PCR functionality, reducing false-negative results.
Validated instruments include ABI 7500, LightCycler 480 II, Mx3005P, QuantStudio 5/6/7, qTOWER3G, MIC, and cobas z 480.
Primers and probes were validated against public databases to ensure detection of BVDV strains while avoiding cross-reactivity.
BactoReal®, MycoReal®, ParoReal® and ViroReal® Kits run with the same thermal cycling conditions.