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| Product Manual ViroReal® Kit Aparavirus |
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| Material Safety Data Sheet ViroReal® Kit Aparavirus |
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Background: Aparaviruses are positive single-stranded RNA viruses within the family Dicistroviridae. Three species within the genus Aparavirus are closely related and summarized in the so-called AKI-complex: Acute bee paralysis virus (ABPV), Kashmir bee virus (KBV) and Israeli acute paralysis virus (IAPV). That complex plays a role in the sudden collapses of honeybee hives and colonies and is responsible for immense economical losses worldwide. Together with other environmental factors they are involved in the colony collapse disorder (CCD). Honeybees, bumblebees and other invertebrates could be infected also persistently. The ways of transmission are horizontal and vertical, through contamination of faeces and saliva, in royal jelly e.g.. Viruses are also found in mites, that act as an ectoparasitic vector. Stress and reduced immunity due to infestation with Varroa could activate the replication of the virus. That leads to mortality of the offspring, weaken the performance and shorten the lifespan of adults immensely.
To distinguish between the Aparavirus species within the AKI-complex, ingenetix also offers individual real-time PCR Kits for detection of Acute bee paralysis virus, Kashmir bee virus and Israeli acute paralysis virus.
Description: ViroReal® Kit Aparavirus is a real-time PCR for the qualitative detection of the RNA of the capsid protein gene of Aparaviruses. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DVEV03913 | 100 | FAM | Cy5 | capsid protein gene |
| DVEV03953 | 50 | FAM | Cy5 | capsid protein gene |
Shipping Temperature: -20 °C dry ice
- Amplification and detection: RNA of capsid protein gene of Aparaviruses
- Transcription with thermostable MMLV Reverse Transcriptase
- Contains RNase Inhibitor to block RNA degradation
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR platforms: runs on all established standard real-time PCR platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Available formats: Kit (with reaction mix)
PCR platforms: ViroReal® Kit Aparavirus was validated with the QuantStudio™ 5 Real-Time PCR System (Thermo Fisher Scientific) and tested with the QuantStudio™ 7 Real-Time PCR System (Thermo Fisher Scientific). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in FAM and Cy5 channel (e.g. ABI® 7500 Fast Real-Time PCR System (Thermo Fisher Scientific), Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) und cobas z 480 Analyzer (Roche), QuantStudio™ 6 (Thermo Fisher Scientific)).