Background:  Aparaviruses are positive single-stranded RNA viruses within the family Dicistroviridae. Three species within the genus Aparavirus are closely related and summerized in the so-called AKI-complex: Acute bee paralysis virus (ABPV), Kashmir bee virus (KBV) and Israeli acute paralysis virus (IAPV). That complex plays a role in the sudden collapses of honeybee hives and colonies and is responsible for immense economical losses worldwide. Together with other environmental factors they are involved in the colony collapse disorder (CCD). Honeybees, bumblebees and other invertebrates could be infected also persistently. The ways of transmission are horizontal and vertical, through contamination of faeces and saliva, in royal jelly e.g.. Viruses are also found in mites, that act as an ectoparasitic vector. Stress and reduced immunity due to infestation with Varroa could activate the replication of the virus. That leads to mortality of the offspring, weaken the performance and shorten the lifespan of adults immensely.
To distinguish between the Aparavirus species within the AKI-complex, ingenetix also offers individual real-time PCR Kits for detection of Acute bee paralysis virus, Kashmir bee virus and Israeli acute paralysis virus.

Description:  ViroReal® Kit Aparavirus is a real-time PCR for the qualitative detection of the RNA of the capsid protein gene of Aparaviruses. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

ViroReal® Kit Aparavirus
Order Number Reactions Channel Pathogen Channel IPC Target
DVEV03913 100 FAM Cy5 capsid protein gene
DVEV03953 50 FAM Cy5 capsid protein gene
For veterinary use only
Shipping Temperature: -20 °C dry ice
Product Features:
  • Amplification and detection: RNA of capsid protein gene of Aparaviruses
  • Real-time PCR with rapid hot-start Taq DNA polymerase 
  • ROXdye as passive reference 
  • Internal Positive Control System to exclude false-negative results 
  • Optimized to handle PCR inhibitors 
  • PCR- platforms: runs on all established standard real-time PCR- platforms 
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • PCR platforms: ViroReal® Kit Aparavirus was validated with the QuantStudio™ 5 Real-Time PCR System (Thermo Fisher Scientific) and the QuantStudio™ 7 Real-Time PCR System (Thermo Fisher Scientific). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in FAM and Cy5 channel (eg. QuantStudio™ 5/6/7 Real-Time PCR System (Thermo Fisher Scientific), qTOWER3G (Analytik Jena), Mic instrument (bio molecular systems), cobas z 480 Analyzer (Roche)).

    BactoReal®, MycoReal®, ParoReal® and ViroReal® Kits run with the same thermal cycling conditions.