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Product Manual BactoReal® Kit American Foulbrood Download PDF
Material Safety Data Sheet BactoReal® Kit American Foulbrood Download PDF
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Background:  American foulbrood (AFB) is a bacterial brood disease of honeybees caused by the Grampositive and spore-forming bacterium Paenibacillus larvae. The disease is transferred and initiated only by the spore stage of the bacterium. Young bee larvae are infected by spores, then the spores germinate in the gut of the larva and the vegetative form of the bacteria begins to grow, taking its nourishment from the larva. Infected larvae normally die after their cell is sealed. The vegetative form of the bacterium produces many millions of spores, which may remain dormant for many years.

For detection of the​ Paenibacillus larvae genotype ERIC I, ingenetix offers an individual real-time PCR kit.

Description:  BactoReal® Kit American Foulbrood uses real-time PCR for the qualitative detection of the 16S rRNA gene of Paenibacillus larvae. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system.
To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit American Foulbrood
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB05413 100 FAM Cy5 16S rRNA
DVEB05453 50 FAM Cy5 16S rRNA
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: 16S rRNA gene of Paenibacillus larvae
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

    • PCR platforms: BactoReal® Kit American Foulbrood was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), LightCycler® 480 II (Roche Diagnostics) and Mx3005P® (Agilent). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in FAM and Cy5 channel (eg. QuantStudio™ 5/6/7 Real-Time PCR System (Thermo Fisher Scientific), qTOWER3G (Analytik Jena), Mic instrument (bio molecular systems), cobas z 480 Analyzer (Roche)).

    Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

    BactoReal® American Foulbrood
    Order Number Reactions Channel Pathogen Channel IPC Target
    RTGV924 100 FAM Cy5 16S rRNA gene
    For veterinary use only
    Shipping Temperature: -20 °C to +4 °C
    BactoReal®, MycoReal®, ParoReal® and ViroReal® Kits run with the same thermal cycling conditions.