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Product Manual BactoReal® Kit Aerococcus viridans Download PDF
Material Safety Data Sheet BactoReal® Kit Aerococcus viridans Download PDF
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Background:  Aerococcus viridans is a Gram-positive bacterium causing gaffkemia, a fatal lobster disease, and it has been associated with septicemia in sea turtles. Aerococcus viridans has also been isolated from the milk of cows with subclinical mastitis. It can also cause different human infections.

Description:  BactoReal® Kit Aerococcus viridans is a real-time PCR for the qualitative detection of the 16S rRNA gene of Aerococcus viridans. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Aerococcus viridans
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB05313 100 FAM Cy5 16S rRNA
DVEB05353 50 FAM Cy5 16S rRNA
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: 16S rRNA gene of Aerococcus viridans
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Aerococcus viridans was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Aerococcus viridans
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV912 100 FAM Cy5 16S rRNA
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The kit qualitatively detects DNA of Aerococcus viridans by targeting the 16S rRNA gene.
Aerococcus viridans is a Gram-positive bacterium causing gaffkemia in lobsters, septicemia in sea turtles, mastitis in cattle, and opportunistic infections such as endocarditis or meningitis in humans.
The internal positive control (IPC) verifies DNA extraction efficiency and identifies potential PCR inhibition.
The kit is validated for DNA extracts from milk, swabs, and tissues.
The detection limit is 5 DNA copies per PCR reaction.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.