Back
Inquiry

Download

Product Manual ParoReal® Kit Acarapis woodi Download PDF
Material Safety Data Sheet ParoReal® Kit Acarapis woodi Download PDF
Download All

Background:  Acarapis woodi is an endoparasite that colonizes the trachea, respectively the respiratory system of bees. Acarapisosis is listed by the WOAH (World Organisation for Animal Health) as notifiable disease of honeybees and has been found worldwide. Fatal losses of entire apiaries because of high infestation are described. The mite spreads from bee to bee, or through swarming and beekeepers. Infected bees could be asymptomatic or show just a few signs, thus the disease is not easily recognized. Like other arthropod pathogens A. woodi is not easy visible by eyes. In early spring crawling and death bees in front of the hive could be the only sign of infection. Due to this fact and that mites are weakening the immunity of the hives in general, A. woodi might act also as a vector of other bacterial and viral pathogens.

Description:  ParoReal® Kit Acarapis woodi is a real-time PCR test for the qualitative detection of the COX1 gene of Acarapis woodi. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

ParoReal® Kit Acarapis woodi
Order Number Reactions Channel Pathogen Channel IPC Target
DVEP00713 100 FAM Cy5 COX1 gene
DVEP00753 50 FAM Cy5 COX1 gene
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: COX1 gene of Acarapis woodi 
  • Real-time PCR with rapid hot-start Taq DNA polymerase 
  • ROX™ dye as passive reference 
  • Internal Positive Control System to exclude false-negative results 
  • Optimized to handle PCR inhibitors 
  • PCR platforms: runs on all established standard real-time PCR platforms 
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously

PCR platforms: ParoReal® Kit Acarapis woodi was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Frequently Asked Questions:

The kit specifically detects DNA of Angiostrongylus vasorum, the causative agent of canine angiostrongylosis.
Infected dogs may show coughing, dyspnea, neurological signs, bleeding disorders, and in severe cases sudden death.
The IPC confirms proper DNA extraction and identifies potential PCR inhibition, reducing the risk of false-negative results.
The kit has been validated for ABI 7500, LightCycler 480 II, and Mx3005P, and is compatible with other real-time PCR instruments detecting FAM and Cy5 channels.
Primers and probes were carefully designed and validated to detect only Angiostrongylus vasorum without cross-reactivity.
BactoReal®, MycoReal®, ParoReal® and ViroReal® Kits run with the same thermal cycling conditions.