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| Product Manual ParoReal® Kit Ascaris |
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| Material Safety Data Sheet ParoReal® Kit Ascaris |
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Background: Ascaris, also known as roundworm, is a parasitic nematode that causes ascariasis in the small intestine of pigs. Ascaris can infect other animals, such as chickens and cattle and cross-infections between humans and pigs are possible. Roundworms in pigs and humans are considered as two species (A. suum and A. lumbricoides). Female worms can lay up to 200,000 eggs per day. The eggs are covered with a sticky protective coat and can remain infective for years in the soil. In the mild cases, which are most common, the only evidence of the worms is at the time of slaughter where milk spot-like lesions can be seen on the liver ("milk spot"). In heavy cases, which are extremely rare, the worms can block the intestine leading to vomiting, weight loss and death.
Description: ParoReal® Kit Ascaris is a real-time PCR test for the qualitative detection of DNA of the ITS1 region of Ascaris spp. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DVEP00913 | 100 | FAM | Cy5 | ITS1 region |
| DVEP00953 | 50 | FAM | Cy5 | ITS1 region |
Shipping Temperature: -20 °C to +4 °C
- Amplification and detection: DNA of the ITS1 region of Ascaris
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
PCR platforms: ParoReal® Kit Ascaris has been validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific) and was also tested with LightCycler® 480 II (Roche Diagnostics) and Mx3005P® (Agilent). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in FAM and Cy5 channel (eg. QuantStudio™ 5/6/7 Real-Time PCR System (Thermo Fisher Scientific), qTOWER3G (Analytik Jena), Mic instrument (bio molecular systems), cobas z 480 Analyzer (Roche)).