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Product Manual BactoReal® Histophilus somni Download PDF
Material Safety Data Sheet BactoReal® Histophilus somni Download PDF
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Description:  BactoReal® Kit Histophilus somni uses real-time PCR for the detection of the 16S rRMA gene of Histophilus somni. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Histophilus somni
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB06113 100 FAM Cy5 16S rRNA
DVEB06153 50 FAM Cy5 16S rRNA
For veterinary use only
Shipping Temperature: -20 °C to +4 °C

PCR platforms: BactoReal® Kit Histophilus somni is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional -  this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Histophilus somni
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV333 100 FAM Cy5 16S rRNA
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

It detects DNA of Histophilus somni by amplifying a 16S rRNA gene fragment using real-time PCR.
The assay is validated for DNA extracted from tracheal swabs, washes, lung tissue, or reproductive tract material.
The artificial DNA IPC monitors extraction and identifies PCR inhibition and is detected in the Cy5 channel.
The assay detects as few as 10 DNA copies per PCR reaction; LoD95 is 29 copies.
The assay includes only detection reagents; the kit includes all reagents including PCR mix and water.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.