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Product Manual BactoReal® Glaesserella parasuis Download PDF
Material Safety Data Sheet BactoReal® Glaesserella parasuis Download PDF
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Background:  Glaesserella parasuis (former Haemophilus parasuis) is a Gram-negative bacterium (member of the Pasteurellaceae) of the upper respiratory tract of swine. It is the aetiological agent of Glässers disease, a severe systemic disease characterized by fibrinous polyserositis, pericarditis, arthritis and meningitis. In addition to the acute form of the disease,Glaesserella parasuis is an important component of the Porcine Respiratory Disease Complex (PRDC). Fifteen serovars ofGlaesserella parasuis have been reported, but a high percentage of the evaluated isolates are nontypeable. Isolates from the upper respiratory tract are mainly apathogenic strains of clinically healthy swine.

Description:  BactoReal® Kit Glaesserella parasuis uses real-time PCR for the detection of the 16S rRNA gene of Glaesserella parasuis (former H. parasuis). Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Glaesserella parasuis
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB00413 100 FAM Cy5 16S rRNA
DVEB00453 50 FAM Cy5 16S rRNA
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: 16S rRNA gene of Glaesserella parasuis
  • Real-time PCRwith primern, probes and positive control
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously

PCR platforms: BactoReal® Kit Glaesserella parasuis is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Glaesserella parasuis
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV500 100 FAM Cy5 16S rRNA
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

It detects DNA of Glaesserella parasuis by targeting the 16S rRNA gene using real-time PCR.
The assay is validated for DNA extracted from synovial, pleural, pericardial, peritoneal, or cerebrospinal fluid.
The artificial DNA IPC monitors DNA extraction and PCR inhibition and is detected in the Cy5 channel.
Yes, cross-reactions may occur with A. porcinus, A. minor, and A. aphrophilus.
The assay includes only detection reagents; the kit includes a full reagent set including PCR mix and water.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.