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Product Manual BactoReal® European Foulbrood Download PDF
Material Safety Data Sheet BactoReal® European Foulbrood Download PDF
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Background:  European foulbrood (EFB) is a bacterial brood disease of honeybees caused by the Grampositive bacterium Melissococcus plutonius. The disease occurs throughout the world. It may cause serious losses of brood. Larvae are infected by consuming contaminated food fed by the nurse bees. The bacteria multiply within the larval gut, competing with it for its food. Thus, larvae that die from the disease have been starved of food.

Description:  BactoReal® Kit European Foulbrood uses real-time PCR for the qualitative detection of the 16S rRNA gene of Melissococcus plutonius. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit European Foulbrood
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB05513 100 FAM Cy5 16S rRNA
DVEB05553 50 FAM Cy5 16S rRNA
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: 16S rRNA gene of Melissococcus plutonius
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms:

BactoReal® Kit European Foulbrood was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), LightCycler® 480 II (Roche Diagnostics) and Mx3005P® (Agilent). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in FAM and Cy5 channel (eg. QuantStudio™ 5/6/7 Real-Time PCR System (Thermo Fisher Scientific), qTOWER3G (Analytik Jena), Mic instrument (bio molecular systems), cobas z 480 Analyzer (Roche)).

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® European Foulbrood
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV923 100 FAM Cy5 16S rRNA gene
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

It detects DNA of Melissococcus plutonius, the causative agent of European Foulbrood, via amplification of the 16S rRNA gene.
The assay is validated for swab and tissue samples from honeybee larvae suspected of European Foulbrood infection.
An artificial DNA IPC is added during extraction and detected in the Cy5 channel to confirm the absence of inhibition.
The assay detects 5 target copies per reaction. The LoD95 was determined as 36 copies/reaction.
An assay provides detection reagents only, while a kit includes additional components like master mix and positive controls.
BactoReal®, MycoReal®, ParoReal® and ViroReal® Kits run with the same thermal cycling conditions.