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Background: The genus Pestivirus is composed of 4 important pathogens: bovine viral diarrhea virus 1 and 2 (BVDV-1 and BVDV-2), classical swine fever virus (CSFV), and border disease virus of sheep (BDV). Recently, a new putative pestivirus species called “HoBi-like,” “BVDV-3,” or “atypical pestivirus” was identified. The BVD virus is a single-strand, positive-sense RNA virus found throughout the world and causes bovine viral diarrhea (BVD). BVD is a disease of cattle, which causes various clinical symptoms, including diarrhea, mucosal disease, reproductive dysfunctions, and/or hemorrhagic syndrome. Prenatal infection with BVDV results in persistently infected cattle (BVD-PI). These animals carry the virus throughout their lifetime and secrete a large number of viral particles. Old: The genus Pestivirus is composed of four important pathogens: bovine viral diarrhea virus 1 and 2 (BVDV-1 and BVDV-2), classical swine fever virus (CSFV), and border disease virus of sheep (BDV). Recently a new putative pestivirus species called “HoBi-like,” “BVDV-3,” or “atypical pestivirus,” was identified. The BVD virus is a single strain, positive-sense RNA virus found throughout the world and causes bovine viral diarrhoea (BVD). BVD is a disease of cattle, which causes various clinical syndromes including diarrhea, mucosal disease, reproductive dysfunctions and/or hemorrhagic syndrome. Prenatal infection by BVD results in cattles permanently infected (BVD-PI). Such animals carry the virus throughout their lifetime and constantly secrete large numbers of viral particles.
Description: ViroReal® Kit BVD Virus is a real-time PCR for the qualitative detection of the RNA of the 5´UTR of the BVD virus (RNA of BVD virus types BVDV-1, BVDV-2 and BVDV-3). Our carefully designed primers and probes ensure the highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DVEV04613 | 100 | FAM | Cy5 | 5´UTR |
| DVEV04653 | 50 | FAM | Cy5 | 5´UTR |
Shipping Temperature: -20 °C dry ice
- Amplification and detection: 5´UTR of BVD Virus
- Transcription with thermostable MMLV Reverse Transcriptase
- Contains RNase Inhibitor to block RNA degradation
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR platforms: runs on all established standard real-time PCR platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Available formats: Kit (with reaction mix)
- FLI certified
PCR platforms: ViroReal® Kit BVD Virus was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent) and LightCycler® 480 II (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific), qTOWER3G (Analytik Jena), Mic instrument (bio molecular systems), and cobas z 480 Analyzer (Roche)).