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| Product Manual ViroReal® Kit PEDV & SDCV |
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| Material Safety Data Sheet ViroReal® Kit PEDV & SDCV |
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Background: Porcine epidemic diarrhea virus (PEDV), a member of the genus Coronavirus (Alphacoronavirus), family Coronaviridae, is a positive-sense, enveloped, single-stranded RNA virus. Severity of disease is variable and dependent on the epidemiological status of the herd. When epidemic, PEDV causes acute watery diarrhea and vomiting in a large proportion at all ages of swine. If endemic, then diarrhea is observed with lower morbidity but higher mortality rate in suckling and recently weaned pigs. Infected sows and gilts show less reproduction performance. The PED virus is clinically similar to, but antigenically distinct from transmissible gastroenteritis virus (TGEV).
Swine delta coronavirus (SDCV, also called porcine delta coronavirus PDCoV), a member of the genus Coronavirus (Deltacoronavirus), family Coronaviridae, is a positive-sense, enveloped, single-stranded RNA virus. SDCV is a new virus first found in pigs in Hong Kong in 2012 and 2014 in the United States. Swine delta coronavirus causes acute watery diarrhea and vomiting in a large proportion at all ages of swine. Swine delta coronavirus infection is clinically similar to, but genetically distinct from porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV).
Description: ViroReal® Kit PEDV & SDCV is a real-time PCR for the qualitative detection of the nucleocapsid protein gene (N gene) of PEDV (porcine epidemic diarrhea virus) and of the 3’UTR of SDCV (swine delta coronavirus, PDCoV). Our carefully designed primers and probes ensure the highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DVEV01913 | 100 | FAM, VIC | Cy5 | nucleocapsid protein gene |
| DVEV01953 | 50 | FAM, VIC | Cy5 | nucleocapsid protein gene |
Shipping Temperature: -20 °C dry ice
- Amplification and detection: N gene of PEDV and 3’UTR of SDCV
- Transcription with thermostable MMLV Reverse Transcriptase
- Contains RNase Inhibitor to block RNA degradation
- Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle RT-PCR inhibitors
- PCR platforms: runs on all established standard real-time PCR platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
- Available formats: Kit (with reaction mix) or Assay-only
PCR platforms: ViroReal® Kit PEDV & SDCV was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent) and LightCycler® 480 II (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), cobas Z 480 Analyzer (Roche) QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).
Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| RTGV69 | 100 | FAM, VIC | Cy5 | nucleocapsid protein gene |
Shipping Temperature: -20 °C to +4 °C