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Product Manual ViroReal® Kit Porcine Parvovirus 1 Download PDF
Material Safety Data Sheet ViroReal® Kit Porcine Parvovirus 1 Download PDF
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Background:  Porcine parvovirus 1 (PPV 1) is a member of the Parvoviridae family with a single-stranded DNA as genome. PPV is associated with reproductive problems, including abortion, still births, small litters, neonatal deaths and weak piglets. PPV multiplies normally in the intestine of the pig without causing clinical signs. Disease occurs when sero-negative dams are infected in the first half of gestation and the virus crosses the placenta.

Description:  ViroReal® Kit Porcine Parvovirus is a real-time PCR for the qualitative detection of the non-structural protein 1 (NS1) gene of the porcine parvovirus 1 (PPV 1). Our carefully designed primers and probes ensure the highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

ViroReal® Kit Porcine Parvovirus 1
Order Number Reactions Channel Pathogen Channel IPC Target
DVEV00713 100 FAM Cy5 non-structural protein 1
DVEV00753 50 FAM Cy5 non-structural protein 1
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: NS1 gene of Porcine Parvovirus 1
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ViroReal® Kit Porcine Parvovirus 1 was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent) and LightCycler® 480 II (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific), qTOWER3G (Analytik Jena), Mic instrument (bio molecular systems), cobas z 480 Analyzer (Roche)).

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® Porcine Parvovirus 1
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV010 100 FAM Cy5 non-structural protein 1
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The kit targets the non-structural protein 1 (NS1) gene of PPV1 for specific detection via the FAM channel.
Infected seronegative dams may experience reproductive failure, while infected fetuses may be stillborn, mummified, or born weak.
The DNA IPC verifies DNA extraction quality and detects PCR inhibition, reducing the chance of false-negative results.
Validated systems include ABI 7500, LightCycler 480 II, Mx3005P, QuantStudio 5/6/7, qTOWER3G, MIC, and cobas z 480.
Primers and probes were tested against PCV2 and PRRSV isolates with no observed cross-reactivity, and all PPV1 strains were successfully detected.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.