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Product Manual ParoReal® Kit Cryptosporidium parvum Download PDF
Material Safety Data Sheet ParoReal® Kit Cryptosporidium parvum Download PDF
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Background:  Cryptosporidium parvum is one of several protozoal species that cause cryptosporidiosis, a parasitic disease of the mammalian intestinal tract. It is a zoonotic and obligate intracellular parasite. Primary symptoms of C. parvum infection are acute, watery, and non-bloody diarrhea. C. parvum infection is of particular concern in immunocompromised patients. Extra-intestinal sites include the lung, liver and gall bladder where it causes respiratory cryptosporidosis, hepatitis and cholecystitis. Infection is caused by ingestion of sporulated oocysts transmitted by the faecal-oral route.

Description:  ParoReal® Kit Cryptosporidium parvum uses is a real-time PCR for the qualitative detection of the GP60 gene of Cryptosporidium parvum. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

ParoReal® Kit Cryptosporidium parvum
Order Number Reactions Channel Pathogen Channel IPC Target
DVEP00613 100 FAM Cy5 GP60
DVEP00653 50 FAM Cy5 GP60
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: GP60 gene of Cryptosporidium parvum
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ParoReal® Kit Cryptosporidium parvum was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ParoReal® Cryptosporidium parvum
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV270 100 FAM Cy5 GP60
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The kit amplifies the GP60 gene of Cryptosporidium parvum for specific detection.
Typical symptoms include acute, watery, non-bloody diarrhea; in severe cases, infection may spread to the liver, lungs, or gall bladder.
The IPC identifies failures in DNA extraction or PCR inhibition, ensuring reliable test interpretation.
The kit has been validated on ABI 7500, LightCycler 480 II, and Mx3005P, and is compatible with instruments detecting FAM and Cy5 channels.
Primers and probes were designed and validated to detect Cryptosporidium parvum specifically, with no cross-reactivity to other species except C. felis.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.