Back
Inquiry

Download

Product Manual MycoReal® Kit Ascosphaera apis Download PDF
Material Safety Data Sheet MycoReal® Kit Ascosphaera apis Download PDF
Download All

Background:  Ascosphaera apis is a fungus belonging to the family Ascophaeraceae and affecting honeybee brood. This fungus causes the larval disease chalkbrood of honeybees (Apis mellifera). Larvae get infected by ingesting fungal spores that germinate within the digestive tract and compete with the larvae for food leading to starvation. The fungus consumes the body of the larvae, resulting in a white and “chalky” appearance, hence the designation chalkbrood, and is most visible during wet springs with cooler outside temperatures. The chalky mummies are highly infectious and spores of this fungus often re-infect colonies. Ascosphaera apis causes large economic losses, particularly in combination with other pathogens.

Description:  MycoReal® Kit Ascosphaera apis is a real-time PCR for the qualitative detection of the ITS1 region of Ascosphaera apis. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

MycoReal® Kit Ascosphaera apis
Order Number Reactions Channel Pathogen Channel IPC Target
DVEF00213 100 FAM Cy5 ITS1 region
DVEF00253 50 FAM Cy5 ITS1 region
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: ITS1 region of Ascosphaera apis
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: MycoReal® Kit Ascosphaera apis was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

MycoReal® Ascosphaera apis
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV932 100 FAM Cy5 ITS1 region
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The kit amplifies the ITS1 region of Ascosphaera apis for specific detection.
Infected larvae develop a white, chalky appearance and die, leading to infectious “mummies” that spread the disease within the hive.
The IPC verifies successful DNA extraction and rules out inhibition of the PCR reaction.
The kit has been validated on ABI 7500, LightCycler 480 II, and Mx3005P, and is compatible with other instruments detecting FAM and Cy5 channels.
Primers and probes were designed and validated to specifically detect Ascosphaera apis without cross-reactivity to other fungi.
BactoReal®, MycoReal®, ParoReal® and ViroReal® Kits run with the same thermal cycling conditions.