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Product Manual BactoReal® Kit Riemerella anatipestifer Download PDF
Material Safety Data Sheet BactoReal® Kit Riemerella anatipestifer Download PDF
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Background:  Riemerella anatipestifer is a Gram-negative bacterium that causes acute or chronic septicaemia. It primarily affects young ducks and less frequently turkeys and geese throughout the world. There are 21 known serotypes and infection is spread horizontally between birds. Infection may be referred to as Duck Septicaemia, Goose ''flu, Riemerellosis, New Duck Disease and Polyserositis.

Description:  BactoReal® Kit Riemerella anatipestifer is a real-time PCR for the qualitative detection of the 16S-23S ribosomal RNA intergenic spacer of Riemerella anatipestifer. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Riemerella anatipestifer
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB05913 100 FAM Cy5 16S-23S ribosomal RNA intergenic spacer
DVEB05953 50 FAM Cy5 16S-23S ribosomal RNA intergenic spacer
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: 16S-23S rRNA spacer of Riemerella anatipestifer
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Riemerella anatipestifer was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Riemerella anatipestifer
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV232 100 FAM Cy5 16S-23S ribosomal RNA intergenic spacer
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The kit amplifies the 16S–23S rRNA intergenic spacer region, with R. anatipestifer DNA detected in the FAM channel.
Infected birds may show septicaemia, fibrinous polyserositis, and high mortality, especially in ducklings.
Pathogen DNA is detected in the FAM channel and the IPC is detected in the Cy5 channel.
Positive and negative controls along with the IPC guarantee accurate and interpretable results.
The kit was validated with ABI 7500, LightCycler® 480 II, and Mx3005P®, and is compatible with other systems detecting FAM and Cy5.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.