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Product Manual BactoReal® Kit European Foulbrood Download PDF
Material Safety Data Sheet BactoReal® Kit European Foulbrood Download PDF
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Background:  European foulbrood (EFB) is a bacterial brood disease of honeybees caused by the Gram-positive bacterium Melissococcus plutonius. The disease occurs throughout the world. It may cause serious losses of brood. Larvae are infected by consuming contaminated food fed by the nurse bees. The bacteria multiply within the larval gut, competing with it for its food. Thus, larvae that die from the disease have been starved of food.

Description:  BactoReal® Kit European Foulbrood is a real-time PCR for the qualitative detection of the 16S rRNA gene of Melissococcus plutonius. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit European Foulbrood
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB05513 100 FAM Cy5 16S rRNA
DVEB05553 50 FAM Cy5 16S rRNA
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: 16S rRNA gene of Melissococcus plutonius
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit European Foulbrood was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® European Foulbrood
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV923 100 FAM Cy5 16S rRNA gene
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The kit amplifies the 16S rRNA gene, with pathogen DNA detected in the FAM channel and the IPC in the Cy5 channel.
Larvae become infected by consuming contaminated brood food, where the bacteria multiply in the gut and compete with the larvae for nutrients.
The IPC ensures that negative results are not due to failed DNA extraction or PCR inhibition, serving as a control throughout the workflow.
Validated instruments include ABI 7500, LightCycler® 480 II, and Mx3005P®. It is also compatible with other systems detecting FAM and Cy5.
Using separate work areas, sterile consumables, and negative extraction controls are recommended to avoid DNA contamination.
BactoReal®, MycoReal®, ParoReal® and ViroReal® Kits run with the same thermal cycling conditions.