Back
Inquiry

Download

Product Manual BactoReal® Kit Streptococcus spp. Download PDF
Material Safety Data Sheet BactoReal® Kit Streptococcus spp. Download PDF
Download All

Background:  Streptococcus is a genus of Gram-positive bacteria belonging to the Firmicutes and the lactic acid bacteria group. There are currently over 50 species known in this genus. Many streptococcal species are nonpathogenic and form part of the commensal animal or human microbiome of the mouth, skin, intestine, and upper respiratory tract. Certain Streptococcus species are responsible for streptococcal pharyngitis, pink eye, meningitis, bacterial pneumonia, endocarditis, erysipelas, necrotizing fasciitis and bovine mastitis.

Description:  BactoReal® Kit Streptococcus spp. is a real-time PCR for the qualitative detection of the 23S rRNA gene of species of the genus Streptococcus. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Streptococcus spp.
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB03813 100 FAM Cy5 23S rRNA
DVEB03853 50 FAM Cy5 23S rRNA
For research use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: 23S rRNA gene of Streptococcus spp.
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Streptococcus spp. was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Streptococcus spp.
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV904 100 FAM Cy5 23S rRNA
For research use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The kit amplifies the 23S rRNA gene of Streptococcus spp. for specific detection.
Streptococcus species are associated with respiratory infections, endocarditis, skin and soft tissue infections, and mastitis in cattle.
The IPC confirms successful DNA extraction and excludes false negatives due to PCR inhibition.
Streptococcus DNA is detected in the FAM channel, and the IPC is detected in the Cy5 channel.
Primers and probes were validated for specificity; only very high concentrations of unrelated bacteria may cause weak cross-reactions.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.