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Product Manual BactoReal® Coxiella burnetii Download PDF
Material Safety Data Sheet BactoReal® Coxiella burnetii Download PDF
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Background:  Coxiella burnetii (C. burnetii) is a Gram-negative, obligate intracellular, zoonotic bacterial pathogen, which is the causative agent of Q fever (Coxiellosis). The disease is spread through inhalation of a spore-like cell variant or close contact with body fluids. Cattle, sheep and goats represent the main animal reservoir.Seropositivity of ruminants for C. burnetii is associated with abortion, stillbirth and fertility problems. The high resistance to standard disinfectants and environmental influences renders disease control difficult.

Description:  BactoReal® Kit Coxiella burnetii uses real-time PCR for the detection of the IS1111 insertion sequence of Coxiella burnetii. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Coxiella burnetii
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB05813 100 FAM Cy5 IS1111 insertion
DVEB05853 50 FAM Cy5 IS1111 insertion
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: IS1111 of Coxiella burnetii
  • Real-time PCRwith primern, probes and positive control
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously

PCR platforms: BactoReal® Kit Coxiella burnetii is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Coxiella burnetii
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV231 100 FAM Cy5 IS1111 insertion
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The assay qualitatively detects DNA of Coxiella burnetii by amplifying the IS1111 insertion sequence using real-time PCR.
The assay is validated for DNA extracted from tissue and swab samples.
An artificial DNA internal positive control (IPC) is added during extraction and detected in the Cy5 channel to monitor DNA extraction quality and PCR inhibition.
The assay detects at least 10 DNA copies per reaction, equivalent to 0.1–1.0 CFU depending on the strain.
The assay includes only detection reagents and requires an external PCR mix, while the kit provides a complete reagent set including PCR mix and water.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.