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Product Manual BactoReal® Brachyspira pilosicoli Download PDF
Material Safety Data Sheet BactoReal® Brachyspira pilosicoli Download PDF
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Background:  Brachyspira pilosicoli (previously called Serpulina pilosicoli) is an anaerobic intestinal spirochaete. This bacterium colonizes the large intestine of various species, including humans. It is the etiologic agent of human and animal intestinal spirochetosis and rarely implicated as a cause of bacteremia. For example, it is the causative agent of porcine colonic spirochaetosis (PCS). Brachyspira pilosicoli is an anaerobic bacterium but is aerotolerant due, at least in part, to high NADH oxidase activity.

Description:  BactoReal® Kit Brachyspira pilosicoli uses real-time PCR for the detection of the NADH oxidase (nox) gene of B. pilosicoli. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Brachyspira pilosicoli
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB01213 100 FAM Cy5 nox
DVEB01253 50 FAM Cy5 nox
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: nox gene of Brachyspira pilosicoli
  • Real-time PCRwith primern, probes and positive control
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously

PCR platforms: BactoReal® Kit Brachyspira pilosicoli is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Brachyspira pilosicoli
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV620 100 FAM Cy5 nox
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The assay detects DNA of Brachyspira pilosicoli by targeting the nox gene using real-time PCR.
It is validated for DNA extracted from fecal samples and tissue biopsies.
An artificial DNA IPC is added during extraction and detected in the Cy5 channel to monitor extraction quality and PCR inhibition.
The assay detects 10 copies/reaction with an LoD95 of 25 copies per reaction.
The assay contains only detection reagents; the kit includes PCR mix and all necessary components for testing.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.