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Product Manual BactoReal® Brachyspira hyodysenteriae Download PDF
Material Safety Data Sheet BactoReal® Brachyspira hyodysenteriae Download PDF
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Background:  Brachyspira hyodysenteriae (previously called Serpulina hyodysenteriae or Treponema hyodysenteriae) is an anaerobic intestinal spirochaete and the causative agent of swine dysentery. Swine dysentery is a disease characterized by mucohaemorrhagic diarrhoea with lesions confined to the large intestine of pigs. Its incubation time is 10-14 days. The faecal excretion of B. hyodysenteriae starts 2 days after infection. Brachyspira hyodysenteriae is an anaerobic bacterium but is aerotolerant due, at least in part, to high NADH oxidase activity.

Description:  BactoReal® Kit Brachyspira hyodysenteriae uses real-time PCR for the detection of the NADH oxidase (nox) gene of B. hyodysenteriae. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Brachyspira hyodysenteriae
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB01113 100 FAM Cy5 nox
DVEB01153 50 FAM Cy5 nox
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: nox gene of Brachyspira hyodysenteriae
  • Real-time PCRwith primern, probes and positive control
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously

PCR platforms: BactoReal® Kit Brachyspira hyodysenteriae is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Brachyspira hyodysenteriae
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV610 100 FAM Cy5 nox
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The assay detects DNA of Brachyspira hyodysenteriae by targeting the nox gene using real-time PCR.
It is validated for DNA extracted from fecal samples and tissue biopsies.
The IPC verifies DNA extraction and detects PCR inhibition. It is added during lysis and detected in the Cy5 channel.
The analytical sensitivity is 10 copies per reaction; LoD95 was determined to be 18 copies.
The assay contains only detection reagents, while the kit includes a full reagent set including PCR mix and water.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.