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Product Manual BactoReal® Kit Brachyspira hyo/pilo Download PDF
Material Safety Data Sheet BactoReal® Kit Brachyspira hyo/pilo Download PDF
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Background:  Brachyspira are anaerobic intestinal spirochaetes. Brachyspira hyodysenteriae (previously called Serpulina hyodysenteriae or Treponema hyodysenteriae) is the causative agent of swine dysentery. Swine dysentery is a disease characterized by mucohaemorrhagic diarrhoea with lesions confined to the large intestine of pigs. Its incubation time is 10-14 days. The faecal excretion of B. hyodysenteriae starts 2 days after infection. Brachyspira pilosicoli (previously called Serpulina pilosicoli) colonizes the large intestine of various species, including humans. It is the etiologic agent of human and animal intestinal spirochetosis and rarely implicated as a cause of bacteremia. For example, it is the causative agent of porcine colonic spirochaetosis (PCS). Brachyspira are anaerobic bacteria but are aerotolerant due, at least in part, to high NADH oxidase activity.

Description:  BactoReal® Kit Brachyspira hyo/pilo uses real-time PCR for the detection of the NADH oxidase (nox) gene of B. hyodysenteriae and B. pilosicoli. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG) to minimize PCR cross-contamination.

BactoReal® Kit Brachyspira hyo/pilo
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB01813 100 FAM Cy5 nox
DVEB01853 50 FAM Cy5 nox
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: NADH nox gene of B. hyodysenteriae and B. pilosicoli
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: BactoReal® Kit Brachyspira hyo/pilo is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Brachyspira hyo/pilo
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV600 100 FAM Cy5 nox
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.