Background:  Noroviruses are non-enveloped, single-stranded, positive-sense RNA viruses ((+)ssRNA), belonging to the family Caliciviridae. Strains are classified in five genotypes (GI – GV), infecting different species, including human, bovine, swine, mice and oysters. Bovine norovirus belong to GIII. Norovirus GIII includes two genotypes (GIII.1 and GIII.2), with genotype GIII.1 represented by jena virus and GIII.2 represented by newbury-2 virus. Infection is associated with gastrointestinal manifestations such as diarrhea, villus atrophy and crypt hyperplasia of the proximal small intestine in cattle, especially in young calves.

Description:  ViroReal® Kit Bovine Norovirus uses one-step reverse transcription RT real-time PCR for the detection of ORF1 of so far known bovine norovirus genotypes GIII.1 and GIII.2. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.

ViroReal® Kit Bovine Norovirus
Order Number Reactions Channel Pathogen Channel IPC Target
DVEV02613 100 FAM Cy5 ORF 1
DVEV02653 50 FAM Cy5 ORF 1
Shipping Temperature: -20 °C dry ice
Product Features:
  • Amplification and detection: ORF1 of Bovine Norovirus
  • Real-time PCRwith primern, probes and positive control
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously

PCR platforms: ViroReal® Kit Bovine Norovirus is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® Bovine Norovirus
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV21 100 FAM Cy5 ORF 1
Shipping Temperature: -20 °C to +4 °C
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.