Background: Noroviruses are non-enveloped, single-stranded, positive-sense RNA viruses ((+)ssRNA), belonging to the family Caliciviridae. Strains are classified in five genotypes (GI – GV), infecting different species, including human, bovine, swine, mice and oysters. Bovine norovirus belong to GIII. Norovirus GIII includes two genotypes (GIII.1 and GIII.2), with genotype GIII.1 represented by jena virus and GIII.2 represented by newbury-2 virus. Infection is associated with gastrointestinal manifestations such as diarrhea, villus atrophy and crypt hyperplasia of the proximal small intestine in cattle, especially in young calves.

Description: ViroReal® Kit Bovine Norovirus uses one-step reverse transcription RT real-time PCR for the detection of ORF1 of so far known bovine norovirus genotypes GIII.1 and GIII.2. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well. For more detailed information see here.

ViroReal® Kit Bovine Norovirus
OrdernumberReactionsChannel PathogenChannel IPCTarget
For veterinary use only.
Shipping Temperature: -20°C dry ice
Product Features:
  • Amplification and detection: ORF1 of Bovine Norovirus
  • Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
  • contains RNase Inhibitor to block RNA degradation
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle RT-PCR inhibitors
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously
  • Available formats: Kit (with reaction mix) or Assay-only

PCR platforms: ViroReal® Kit Bovine Norovirus is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® Bovine Norovirus
OrdernumberReactionsChannel PathogenChannel IPCTarget
For veterinary use only.
Shipping Temperature: +4°C cool packs
BactoReal®, MycoReal®, ParoReal® and ViroReal® Kits run with the same thermal cycling conditions.