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Product Manual BactoReal® Bordetella bronchiseptica Download PDF
Material Safety Data Sheet BactoReal® Bordetella bronchiseptica Download PDF
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Background:  Bordetella bronchiseptica is a Gram-negative bacterium found as a normal inhabitant of the respiratory tract in a variety of animals such as dogs, pigs, some rodents, as well as humans. In some cases, it causes pneumonia, canine infectious tracheobronchitis (kennel cough), bronchopneumonia in cats, and atrophic rhinitis in pigs. It is widely distributed in the swine population. Here, some strains cause a mild and non-progressive rhinitis that heals spontaneously. However if toxigenic pasteurella are present in the herd then a combination of the two organisms can produce severe progressive rhinitis (PAR). Bordetella bronchiseptica can also be a secondary opportunist invader in pneumonia.

Description:  BactoReal® Kit Bordetella bronchiseptica uses real-time PCR for the detection of the bfrA gene of Bordetella bronchiseptica. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Bordetella bronchiseptica
Order Number Reactions Channel Pathogen Channel IPC Target
DVEB02413 100 FAM Cy5 bfrA gene
DVEB02453 50 FAM Cy5 bfrA gene
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: bfrA gene of Bordetella bronchiseptica
  • Real-time PCRwith primern, probes and positive control
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously

PCR platforms: BactoReal® Kit Bordetella bronchiseptica is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

BactoReal® Bordetella bronchiseptica
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV1250 100 FAM Cy5 bfrA gene
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The assay qualitatively detects DNA of Bordetella bronchiseptica by targeting the bfrA gene.
The assay is validated for DNA extracted from respiratory tract samples.
An artificial DNA internal positive control (IPC) is added during extraction and detected in the Cy5 channel to monitor extraction quality and PCR inhibition.
The analytical sensitivity is 1 DNA copy per PCR reaction, allowing highly sensitive detection.
The assay contains only detection reagents and requires an external PCR mix, while the kit provides a complete reagent set including PCR mix and water.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.