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Product Manual ViroReal® Bluetongue Virus (BTV) Download PDF
Material Safety Data Sheet ViroReal® Bluetongue Virus (BTV) Download PDF
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Background:  Bluetongue virus (BTV) is a segmented, linear, double-stranded RNA virus (dsRNA), belonging to the Reoviridae family and is divided into 28 serotypes. The virus is transmitted by biting midges and infects wild and domestic ruminants such as sheep and less frequently goats, deer and cattle. There is no appropriate treatment of acute disease, which is associated with high morbidity and mortality in some sheep breeds. Prophylactic immunisation with attenuated and inactivated vaccines is available. However, they do not cover all virulent BTV serotypes.

Description:  ViroReal® Kit Bluetongue Virus (BTV) uses one-step reverse transcription RT real-time PCR for the detection of the segment 10 (NS3) of BTV. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). Reverse transcription and PCR can be performed in one reaction well.

ViroReal® Kit Bluetongue Virus (BTV)
Order Number Reactions Channel Pathogen Channel IPC Target
DVEV02213 100 FAM Cy5 NS3
DVEV02253 50 FAM Cy5 NS3
For veterinary use only
Shipping Temperature: -20 °C dry ice
Product Features:
  • Amplification and detection: NS3 gene of Bluetongue Virus (BTV)
  • Real-time PCRwith primern, probes and positive control
  • PCR- platforms: runs on all established standard real-time PCR- platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously

PCR platforms: ViroReal® Kit Bluetongue Virus (BTV) is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.

Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.

ViroReal® Bluetongue Virus (BTV)
Order Number Reactions Channel Pathogen Channel IPC Target
RTGV22 100 FAM Cy5 NS3
For veterinary use only
Shipping Temperature: -20 °C to +4 °C
Frequently Asked Questions:

The assay qualitatively detects RNA of Bluetongue Virus (BTV) by targeting segment 10 (NS3 gene).
The assay is validated for RNA extracted from tissue samples (e.g., spleen, lymph nodes) and blood of ruminants.
An artificial RNA internal positive control (IPC) is added during extraction and detected in the Cy5 channel to confirm extraction success and detect PCR inhibition.
The assay reliably detects at least 50 target RNA copies per reaction and is linear from 100 to 1,000,000 copies.
The assay contains only detection reagents and requires an external PCR mix, while the kit provides a complete reagent set including PCR mix and water.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.