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Product Manual BactoReal® Kit Corynebacterium pseudotuberculosis | Download PDF |
Material Safety Data Sheet BactoReal® Kit Corynebacterium pseudotuberculosis | Download PDF |
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Background: Corynebacterium pseudotuberculosis is a Grampositive bacterium and the aetiological agent of external or cutaneous abscessation, lymphangitis, and internal abscessation affecting sheep, goats, horses, cattle and rarely humans as well. Corynebacterium pseudotuberculosis produces an exotoxin called phospholipase D that enhances dissemination of the bacteria by damaging endothelial cells and increasing vascular permeability.
Description: BactoReal® Kit Corynebacterium pseudotuberculosis uses real-time PCR for the detection of the phosholipase D (pld) gene of Corynebacterium pseudotuberculosis. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).
Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
---|---|---|---|---|
DVEB05213 | 100 | FAM | Cy5 | pld |
DVEB05253 | 50 | FAM | Cy5 | pld |
Shipping Temperature: -20 °C to +4 °C
- Amplification and detection: pld gene of Corynebacterium pseudotuberculosis
- Real-time PCRwith primern, probes and positive control
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
PCR platforms: BactoReal® Kit Corynebacterium pseudotuberculosis is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.
Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.
Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
---|---|---|---|---|
RTGV919 | 100 | FAM | Cy5 | pld |
Shipping Temperature: -20 °C to +4 °C