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Product Manual BactoReal® Chlamydophila psittaci/abortus | Download PDF |
Material Safety Data Sheet BactoReal® Chlamydophila psittaci/abortus | Download PDF |
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Background: Chlamydophila psittaci (previously classified as Chlamydia psittaci) is an intracellular bacterium that may cause endemic avian chlamydiosis in feral birds and domesticated poultry, epizootic outbreaks in mammals such as cattle, pigs, sheep and horses, and respiratory psittacosis in humans (also known as parrot fever and ornithosis). It is transmitted by inhalation, contact or ingestion among birds and to mammals. Chlamydophila psittaci is present in feces, nasal secretions, and feathers of infected birds. Chlamydophila abortus (previously classified as Chlamydia psittaci serotype 1) is one of the major infectious causes of abortion in sheep, cattle and goats worldwide by colonizing the placenta. Enzootic abortion in sheep is also known as ovine enzootic abortion (OEA) or enzootic abortion of ewes (EAE).
Description: BactoReal® Kit Chlamydophila psittaci/abortus uses real-time PCR for the detection of the omp1 gene of C. psittaci and C. abortus. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC). To minimize PCR cross-contamination,the reaction mix included contains dUTP and uracil-N glycosylase (UNG).
Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
---|---|---|---|---|
DVEB03013 | 100 | FAM | Cy5 | omp1 gene |
DVEB03053 | 50 | FAM | Cy5 | omp1 gene |
Shipping Temperature: -20 °C to +4 °C
- Amplification and detection: omp1 gene of Chlamydophila psittaci and Chlamydophila abortus
- Real-time PCRwith primern, probes and positive control
- PCR- platforms: runs on all established standard real-time PCR- platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
PCR platforms: BactoReal® Kit Chlamydophila psittaci/abortus is developed and validated for the Applied Biosystems® 7500 System (Thermo Fisher Scientific), LightCycler® 480 (Roche) and Mx3005P® QPCR System (Agilent), but is also suitable for other real-time PCR instruments.
Assay: Optional - this product is also available as an assay-only format with primers, probe and a positive control, but without reaction mix.
Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
---|---|---|---|---|
RTGV1410 | 100 | FAM | Cy5 | omp1 gene |
Shipping Temperature: -20 °C to +4 °C