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Product Manual BactoReal® Kit Tropheryma whipplei Download PDF
Material Safety Data Sheet BactoReal® Kit Tropheryma whipplei Download PDF
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Background:  Tropheryma whipplei is a gram-positive bacterium that it is very difficult to cultivate. It is a human pathogen causing Whipple''s disease, which is a rare, chronic, systemic illness typically affecting white, middle-aged men. It causes intestinal malabsorption that leads to cachexia. In the majority of cases, multiple organ systems are affected. Symptoms include diarrhea and abdominal pain, joint pain, arthritis, and even dementia. It manifests in the body in various forms and can go undetected for decades before suddenly causing a fatal illness.

Description:  BactoReal® Kit Tropheryma whipplei is a real-time PCR for the qualitative detection of the groEL gene of Tropheryma whipplei. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Tropheryma whipplei
Order Number Reactions Channel Pathogen Channel IPC Target
DHUB01653 50 FAM Cy5 groEL gene
For research use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: groEL gene of Tropheryma whipplei
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously

PCR platforms: BactoReal® Kit Tropheryma whipplei was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Frequently Asked Questions:

The bacterium Tropheryma whipplei is the causative agent of Whipple’s disease, a rare multisystemic infection that can affect the intestine, central nervous system, and other organs.
The kit uses primers and probes directed against unique repeated sequences of Tropheryma whipplei, minimizing cross-reactivity with related bacterial species.
Yes, the real-time PCR technology combined with optimized reagents allows sensitive detection even in samples with very low bacterial concentrations.
Yes, cerebrospinal fluid is one of the validated sample types, which is particularly important in suspected neurological manifestations of Whipple’s disease.
The internal control monitors nucleic acid extraction and PCR amplification, ensuring reliable results by identifying false negatives due to inhibition.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.