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Product Description BactoReal® Kit Streptococcus pneumoniae Download PDF
Product Manual BactoReal® Kit Streptococcus pneumoniae Download PDF
Material Safety Data Sheet BactoReal® Kit Streptococcus pneumoniae Download PDF
Declaration of Conformity BactoReal® Kit Streptococcus pneumoniae Download PDF
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Background:  Streptococcus pneumoniae (pneumococci) is a Gram-positive, alpha-hemolytic bacterium which can cause pneumonia (community-acquired pneumonia, CAP), bronchitis, otitis media, meningitis and sepsis. Pneumococci commonly colonize the upper human respiratory tract and can also be found in healthy persons (carrier rate varies between 40% and 70%). Transmission occurs through close contact with contaminated respiratory droplets. The risk of a pneumococcal infection is much increased in persons with a weak immune system and elder people.

Description:  BactoReal® Kit Streptococcus pneumoniae is a real-time PCR for the qualitative detection of the N-acetylmuramoyl-L-alanine amidase (lytA) gene of Streptococcus pneumoniae. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Streptococcus pneumoniae
Order Number Reactions Channel Pathogen Channel IPC Target
DHUB01453 50 FAM Cy5 lytA gene
For in vitro diagnostic use
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: lytA gene of Streptococcus pneumoniae
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously

PCR platforms: BactoReal® Kit Streptococcus pneumoniae was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific

Frequently Asked Questions:

The kit enables the molecular detection of Streptococcus pneumoniae DNA, supporting accurate diagnosis of pneumococcal infections.
The kit amplifies the lytA gene, which is highly specific for Streptococcus pneumoniae, ensuring precise identification of the pathogen.
Yes, the kit contains an internal control to verify PCR performance and to detect possible reaction inhibition in each sample.
The kit is validated for respiratory samples such as nasopharyngeal swabs, sputum, and bronchoalveolar lavage fluid.
Rapid detection of Streptococcus pneumoniae is essential for early treatment of pneumonia, meningitis, and other invasive infections, helping to reduce morbidity and mortality.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.