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Product Description BactoReal® Kit Mycoplasma pneumoniae Download PDF
Product Manual BactoReal® Kit Mycoplasma pneumoniae Download PDF
Material Safety Data Sheet BactoReal® Kit Mycoplasma pneumoniae Download PDF
Declaration of Conformity BactoReal® Kit Mycoplasma pneumoniae Download PDF
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Background:  Mycoplasma pneumoniae is a very small bacterium lacking a cell wall. This bacterium has a global distribution and is an obligate pathogen and highly infectious. Transmission occurs through close contact with contaminated respiratory droplets. Mycoplasma pneumoniae infects the mucosal membrane of the respiratory tract and is a common cause of both upper and lower respiratory infections, including tracheobronchitis and primary atypical pneumonia (community-acquired pneumonia, CAP), but it can also cause pharyngitis, meningitis and otitis media.

Description:  BactoReal® Kit Mycoplasma pneumoniae is a real-time PCR for the qualitative detection of the 16S rRNA gene of Mycoplasma pneumoniae. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

BactoReal® Kit Mycoplasma pneumoniae
Order Number Reactions Channel Pathogen Channel IPC Target
DHUB01253 50 FAM Cy5 16S rDNA
For in vitro diagnostic use
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: 16S rRNA gene of Mycoplasma pneumoniae
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously

PCR platforms: BactoReal® Kit Mycoplasma pneumoniae was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Frequently Asked Questions:

This kit detects Mycoplasma pneumoniae by targeting its 16S rRNA gene using real‑time PCR (FAM channel).
The kit includes a pathogen-specific assay, an internal DNA positive control assay and target, a DNA reaction mix with dUTP/UNG to prevent contamination, a positive control, and nuclease‑free water.
M. pneumoniae DNA is detected in the FAM channel; the internal positive control (IPC) is detected in the Cy5 channel, allowing verification of DNA extraction and PCR inhibition.
Validated with ABI® 7500 Fast, LightCycler® 480 II, and Mx3005P® systems. Also compatible with any real‑time PCR instrument that supports FAM and Cy5 detection.
The assay detects at least 10 target copies per reaction. The LoD95 is approximately 39 copies/reaction, with high specificity confirmed using bacterial isolates and clinical samples.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.