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Product Manual ViroReal® Kit Mpox Virus Download PDF
Material Safety Data Sheet ViroReal® Kit Mpox Virus Download PDF
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Background:  Monkeypox is a smallpox-like disease caused by the monkeypox virus. Monkeypox virus belongs to the Orthopoxvirus genus. These viruses have a genome of double-stranded DNA and can replicate in the cytosol. The natural reservoir of monkeypox virus are rodents and can be transmitted from them to other animals and to humans. Further transmission from person to person can occur through contact with skin and bodily secretions. Monkeypox virus is endemic in Africa. Two genetically different clades have developed, the West African clade and the Congo Basin (Central Africa) clade. In May 2022 there was an increased incidence of monkeypox virus infections outside of Africa, especially in the US, Canada and Europe. This virus belongs to the West African clade.

Description:  ViroReal® Kit Monkeypox Virus is a real-time PCR for the qualitative detection of the B9R gene of the monkeypox virus, which encodes the interferon-g receptor-like protein. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).

ViroReal® Kit Mpox Virus
Order Number Reactions Channel Pathogen Channel IPC Target
DHUV02753 50 FAM Cy5 B9R
For research use only
Shipping Temperature: -20 °C to +4 °C
Product Features:
  • Amplification and detection: B9R Gen
  • Real-time PCR with rapid hot-start Taq DNA polymerase
  • ROX™ dye as passive reference
  • Internal Positive Control System to exclude false-negative results
  • Optimized to handle PCR inhibitors
  • PCR platforms: runs on all established standard real-time PCR platforms
  • Harmonized thermal profiles to run RNA and DNA samples simultaneously

PCR platforms: ViroReal® Kit Mpox Virus was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).

Frequently Asked Questions:

The kit detects DNA of the monkeypox virus by targeting the B9R gene, with amplification measured in the FAM channel.
Monkeypox is a smallpox-like disease caused by an Orthopoxvirus. It can be transmitted from rodents to humans and between humans, leading to outbreaks that require rapid diagnosis for infection control.
The kit is validated for DNA extracts from clinical specimens processed with recommended extraction methods. It is compatible with multiple sample types when extracted appropriately.
The kit includes an internal DNA positive control (IPC) added during extraction and detected in the Cy5 channel. It monitors DNA extraction efficiency and identifies possible PCR inhibition.
The limit of detection (LoD95%) is 2.2 target copies per reaction. Validation showed linearity from 6 to 6,000,000 copies/reaction and no cross-reactivity with other human DNA viruses.
BactoReal® , MycoReal® , ParoReal ® and ViroReal® Kits run with the same thermal cycling conditions.