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Download All Background: Human metapneumovirus (hMPV) is an enveloped negative single-stranded RNA virus (ss (-)RNA) of the family Paramyxoviridae. It was isolated for the first time in 2001. HMPV is present as two genetic lineages, hMPV A and hMPV B. Human metapneumovirus causes upper and lower respiratory tract infections, symptoms very similar to respiratory syncytial virus (RSV). Human metapneumovirus has a worldwide distribution and it is one of the most common causes of lower respiratory infection in young children. Reinfections appear to be common. Co-infection of human metapneumovirus with influenza virus or RSV can occur.
Description: ViroReal® Kit Metapneumovirus uses is a real-time PCR for the qualitative detection of the nucleocapsid protein gene of human metapneumovirus (hMPV) strains lineages hMPV A and hMPV B. Our carefully designed primers and probe ensure highest sensitivity and specificity. The kit consists of an assay mix for the detection of the pathogen as well as a positive control and an internal positive control (IPC) system. To minimize PCR cross-contamination, the included reaction mix contains dUTP and uracil-N glycosylase (UNG).
| Order Number | Reactions | Channel Pathogen | Channel IPC | Target |
|---|---|---|---|---|
| DHUV01553 | 50 | FAM | Cy5 | nucleocapsid protein gene |
Shipping Temperature: -20 °C dry ice
- Amplification and detection: nucleocapsid protein gene of Metapneumovirus
- Transcription with thermostable MMLV Reverse Transcriptase (M-MLV)
- Contains RNase Inhibitor to block RNA degradation
- CE-IVD Real-time PCR with rapid hot-start Taq DNA polymerase
- ROX™ dye as passive reference
- Internal Positive Control System to exclude false-negative results
- Optimized to handle PCR inhibitors
- PCR platforms: runs on all established standard real-time PCR platforms
- Harmonized thermal profiles to run RNA and DNA samples simultaneously
PCR platforms: ViroReal® Kit Metapneumovirus was validated with the ABI® 7500 Fast Real-Time PCR System (fast cycle parameters are not supported, Thermo Fisher Scientific), and was also tested with Mx3005P® (Agilent), qTOWER3G (Analytik Jena), MIC instrument (bio molecular systems), LightCycler® 480 II (Roche) and cobas Z 480 Analyzer (Roche). It is also compatible with other real-time PCR instruments which detect and differentiate fluorescence in the FAM and Cy5 channel (e.g. QuantStudio™ 5 / 6 / 7 (Thermo Fisher Scientific)).